There are a variety of antivirus software packages that operate in many different ways, depending on how the vendor chose to implement their software. What they have in common, though, is that they all look for patterns in the files or memory of your computer that indicate the possible presence of a known virus. Antivirus packages know what to look for through the use of virus profiles (sometimes called "signatures") provided by the vendor.
New viruses are discovered daily. The effectiveness of antivirus software is dependent on having the latest virus profiles installed on your computer so that it can look for recently discovered viruses. It is important to keep these profiles up to date.
More information about viruses and antivirus software can be found on the CERT Computer Virus Resource page
http://www.cert.org/other_sources/viruses.html
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